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. 2014 May 8;5(11):3512–3525. doi: 10.18632/oncotarget.1953

Fig 6. Restoring miR-152 expression decreases cell proliferation, migration, and invasion.

Fig 6

(a) Proliferation of LNCaP, (b) PC-3, (c) and MDA-PCa2b cells that were transfected with either 30 nM of miR-152 mimic or miR-NC (negative control) was measured by MTT for 6 days. Results shown are representative of three independent experiments averaged and normalized to Day 1 ± s.e. *p<0.05. (d) LNCaP, (e) PC-3, (f) and MDA-PCa-2b cells were analyzed by flow cytometry after 3 days of miR-152 mimic transfection (30 nM). miR-152 caused G2-M arrest in cell cycle progression in PCa cell lines. Results shown are representative of three independent experiments. (g) Relative cell migration of LNCaP PC-3, and MDA-PCa-2b cells was measured utilizing Boyden migration chambers. Results shown are representative of two individual experiments performed in triplicate. (h) LNCaP, PC-3, and MDA-PCa-2b cells transfected with miR-152 mimic showed a decrease in the number of cells invading through a layer of Matrigel relative to cells treated with miR-NC (negative control). All data presented are the means of three independent experiments ± s.e. *P<0.05.