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. 2014 May 7;5(11):3526–3540. doi: 10.18632/oncotarget.1954

Figure 2. 4-ABP-DNA adduct analysis in normal human urothelial mucosa (NHUM) and bladder tumor tissue (BTT) samples.

Figure 2

(A) Chemical structures of three 4-ABP-DNA adducts: 4-ABP-C8-dG, 4-ABP-N2-dG and 4-ABP-C8-dA. The same genomic DNA samples isolated from the normal human urothelial mucosa and bladder tumor tissues that used for acrolein-dG DNA adduct analysis were used for 4-ABP-DNA adduct analysis by the methods described in the text. (B) A typical three dimensional TLC separation of resultant nucleotides from DNA digested with nuclease P1 (NP1). This method is specifically for 4-ABP-N2-dG and 4-ABP-C8-dA adduct analysis. (C) A typical three dimensional TLC result from n-butanol extractions. This method is specifically for 4-ABP-C8-dG adduct analysis. (D) Levels of 4-ABP-DNA adducts in normal human urothelial mucosa [mean ± s.d. = (1.8±0.6) X10−7/dG, n=19] and bladder tumor tissue [mean ± s.d. =(2.1±1.1) × 10−7/dG, n=10], P = 0.32.