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. 2014 May 7;5(11):3526–3540. doi: 10.18632/oncotarget.1954

Figure 4. Relative mutagenicity, mutational signature and mutational spectrum induced by acrolein (Acr)-dG DNA adducts versus 4-ABP-DNA adducts in human urothelial cells.

Figure 4

The supF containing plasmid pSP189 DNA was either reacted with acrolein or N-OH-4-ABP and the DNA adduct number per supercoiled plasmid DNA was determined by a UvrABC incision method (Supplementary Figure S3) [25]. Modified plasmids were transfected into UROtsa cells, mutations in the supF gene were detected, quantified, and sequenced as previously described [25, 31]. (A) Mutation frequency per adduct induced by acrolein-dG DNA adducts and 4-ABP-DNA adducts. (B) Mutational signature and spectrum induced by acrolein-dG DNA adducts and 4-ABP-DNA adducts in the supF gene. Note: acrolein-dG DNA adducts are 5-6 fold more mutagenic than 4-ABP-DNA adducts. Both mutational signature (also see Table 1) and spectrum induced by acrolein-dG DNA adducts and 4-ABP-DNA adducts are different.