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. 2014 May 13;5(11):3636–3650. doi: 10.18632/oncotarget.1970

Figure 3. Sox9 is downstream of Notch signaling in human lung cancer.

Figure 3

(A) Mean mRNA levels and standard deviations of HES1 and SOX9 assessed by quantitative RT-PCR are shown in untreated lung ADC cells (Control), after EDTA-induced Notch activation (EDTA), and after Notch activation was blocked by a gamma secretase inhibitor (EDTA + GSI); *, P < 0.05. (B) mean mRNA fold change and standard deviations of Notch1 and Sox9 are shown in lung adenocarcinoma cells 72 hours after transient transfection with siRNA against Notch1 as compared to transfection with a scrambled siRNA (Control); *, P < 0.05. (C) Expression of Notch1 and Sox9 were analyzed by western blot in lung ADC and immortalized bronchial epithelial cells 48 hours after transfection with vector or Notch1 intracellular domain. Quantification of Sox9 by densitometry, normalized to GAPDH protein levels, is shown.