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. 2014 May 13;5(11):3636–3650. doi: 10.18632/oncotarget.1970

Figure 6. Sox9 mediates Notch1-induced cell invasion and expression of E-cadherin.

Figure 6

(A) invasion assays were performed on H1299 lung ADC cells 48 hours after transient knockdown of Notch1 or Sox9, or overexpression (OE) of Sox9 compared to cells transfected with both a scrambled siRNA sequence and empty vector (Control). Representative images show crystal violet-stained invaded cells. (B) Total number of invaded cells from experiments in A were quantified and compared to the control samples; *, P < 0.05. (C) Intracellular Notch1 (N1ICD), E-cadherin and Sox9 were analyzed by western blot 72 hours after transient knockdown of Notch1 or overexpression of Sox9, or transfection with a scrambled siRNA sequence and empty vector, in H1299 cells. (D) Lung ADC tumors were immunohistochemically stained for Sox9 and E-cadherin. Representative staining is shown in human lung adenocarcinoma samples with high (upper panel) or low (lower panel) Sox9 staining. Scale bar, 100 μm. (E) Quantified Sox9 and E-cadherin immunohistochemical stain intensity scores were graphed; *, P < 0.05.