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. 2014 May 20;5(11):3756–3769. doi: 10.18632/oncotarget.1993

Figure 7.

Figure 7

Nuclear β-catenin expression was suppressed in SW620 cells with shMACC1 compared with the control groups by western blot analysis (A); β-catenin knockdown had no remarkable effect on MACC1 protein expression in SW620 cells transfected with β-catenin-siRNA compared with the control group by western blot analysis (B). GAPDH level was considered loading control, Histone3 was considered as nuclear loading control. MACC1 expression significantly increased β-catenin transcriptional activity in SW620 cells transfected with MACC1 expression plasmid at 48 hours compared with the empty vector control group by dual-luciferase reporter assay (p=0.014; C). MACC1 expression increased Met, c-Myc, cyclin D1, MMP9, phos-GSK-3β (Ser9), and vimentin expression, but suppressed cleaved caspase-3 and E-cadherin expression in HCT116 cells transfected with MACC1 over-expression plasmid compared with the control group by western blot analysis. However, MACC1 knockdown reversed all these changes in SW620 cells by western blot analysis (D).