Figure 2. CTGF increases cell migration by suppressing miR-519d expression.
(A) The miR-519d expression in indicated cells was examined by qRT-PCR. (B) MG-63 cells and U-2 OS cells were incubated with rCTGF (5-50 ng/ml) for 24 h, miR-519d expression examined by qRT-PCR. (C) Cells were transfected with miR-519d mimic or inhibitor for 24 h and cell migration examined by Transwell assay. Results are expressed as mean ± SEM. *, p < 0.05 as compared with controls, and #, p < 0.05 as compared with CTGF-treated control group.
