Fig. 3.
Effect of mutations in Tn on velocity-pCa relationship at different pH. VRTF determined at normal (7.4) and low pH (6.8) using filaments reconstituted with WT Tn, Tn with a V43Q mutation in TnC, and Tn with TnI truncated beyond R156. VRTF plotted as a function of the -log of the [Ca++] (pCa). Data points represent means ± SE. Data were fit with Hill equation with fit parameters displayed in Table 1. *VRTF with V43Q is significantly different from control at the given pCa value. #VRTF with R156 is significantly different from control at the given pCa value.