Figure 3.

51KO cells are resistant to lipid accumulation and have elevated Dex-induced lipolysis. A, Western blot analysis of WT and 51KO MEFs demonstrating a complete lack of FKBP51 in the 51KO cells and restoration of FKBP51 expression after rescue expression of FLAG-FKBP51 (KO-R). Hsp90 was used as loading control. B, Detection of lipid accumulation in undifferentiated (U) and differentiated (D) WT, 51KO, and KO-R cells by Nile Red staining. Phase-contrast images are also shown. C, Quantitation of intracellular lipid in cells treated in panel B by optical density (±SEM; n = 3–6). D, FAS enzymatic activity in differentiated WT and 51KO cells (±SEM; n = 6). E, Nile Red staining of lipid content in WT and 51KO cells subjected to Dex treatment every medium change during differentiation (Dex ET). F, Quantitation of intracellular lipid in cells treated in panel E by optical density (±SEM; n = 6). Note the reduced y-axis range compared with that in panel C. Statistical differences are indicated as follows: *, P > .05; **, P > .01; ***, P > .001; the same parameters apply for # and ∧ symbols. *, WT vs WT; #, 51KO vs 51KO; ∧, WT vs 51KO.