Interaction of FKBP51 with GRα and PPARγ. GRα and PPARγ transfected COS-7 cells were adsorbed to protein G-Sepharose using the FiGR monoclonal antibody to GRα (I), PPARγ monoclonal antibody (I), or nonimmune IgG (NI), followed by Western blotting for GRα, PPARγ, Hsp90, and FKBP51 proteins. A representative of 3 independent experiments is shown.