Skip to main content
. 2014 Jun 16;28(8):1254–1264. doi: 10.1210/me.2014-1023

Figure 4.

Figure 4.

FKBP51 regulates Akt phosphorylation at serine 473 and p38 MAPK activation. A, WT and 51KO MEFs treated with or without insulin (INS) for 30 minutes were analyzed by Western blotting with Akt antibodies specific to phosphoserine 473 or total Akt. B, WT and 51KO MEFs treated with or without insulin for 30 minutes were analyzed by Western blotting with a p38 antibody with dual specificity against phosphothreonine 180/phosphotyrosine 182 or an antibody against total p38 MAPK. C, WT and 51KO MEFs treated with or without Dex (100 nM) for 1 hour were analyzed by Western blotting with the p38 antibodies (see panel B). D, WT and 51KO MEFs treated with or without Dex for 1 hour were analyzed by Western blotting with Akt antibodies (see panel A). All quantitations were performed by infrared spectrophotometry and phospho-Akt levels were normalized to total Akt while phospho-p38 levels were normalized to total p38. Hsp90 was used as a loading control. All results represent means ± SEM (n = 4). Statistical differences are indicated as follows: *, P > .05; **, P > .01; ***, P > .001; the same parameters apply for # and ∧ symbols. *, WT vs WT; #, 51KO vs 51KO; ∧, WT vs 51KO.