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. 2014 Jun 16;28(8):1254–1264. doi: 10.1210/me.2014-1023

Figure 5.

Figure 5.

Insulin reciprocally regulates the intrinsic transcriptional activities of GRα and PPARγ. A, COS-7 cells were transiently transfected with GRα and GRE-Luc reporter in the absence or presence of insulin and Dex, as indicated. B, Similarly, COS-7 were transiently transfected with PPARγ and PPRE-Luc in the absence or presence of insulin and Rosi, as indicated. In each case, cells were transfected with pRL-CMV-Renilla to normalize for transfection efficiency. Final luciferase values were normalized to 1 for untreated cells. Results represent the means ± SEM (n = 6). *, control vs insulin; #, Dex or Rosi vs Dex or Rosi + insulin. Significant differences for all data are indicated as follows: *, P > .05; **, P > .01; the same parameters apply for #.