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. Author manuscript; available in PMC: 2014 Jul 31.
Published in final edited form as: Immunobiology. 2011 Aug 27;217(1):28–36. doi: 10.1016/j.imbio.2011.08.008

Figure 4. T. gondii mediated inhibition of MHC-class II and ICAM1 occurs in infected and non-infected cells independent of cell invasion.

Figure 4

(A) RAW264.7 cells were infected with viable RH, Mic8 knockout (grown in presence of ATc) or ME49 parasites or RH parasites that were inhibited with1 μM Cytochalasin D or killed (three freeze/thaw cycles, f/t) at a MOI 1. Cells were stimulated with 50 ng/ml IFNγ and analyzed after overnight incubation for expression of MHC-class II molecules by flowcytometry (one out of at least three experiments is shown). Similar experiments as in (A) were performed for different parasite:host cell ratios and mean fluorescence intensities of MHC-class II (B) or ICAM1 (C) expression were determined for either uninfected (GFP−) or infected (GFP+) cells (mean+SEM, N=4).