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. 2014 Jul 31;4:5896. doi: 10.1038/srep05896

Figure 4. Proteasome inhibition induces mitochondrial oxidation and ubiquitinated protein accumulation before cytosol is oxidized.

Figure 4

(A) Time course of accumulation of polyubiquitinated proteins in the light mitochondria fraction (LMF) and (B) time-lapse measurement of the redox state in the mitochondria (left panels) and cytosol (right panels) using mitochondria or cytosolic-targeted roGFP after treatment with DMSO (control) or 10 μM MG132. VDAC1, an outer membrane mitochondrial protein, was used as a mitochondrial loading control, and actin was used as a cytosolic loading control.