Table 3.

Δ(ΔG) values for 138 hydrophobic variants from 11 proteins.^a

Varianta	Volumeb (Å3)	ΔGtr to Octanolc	ΔGtr to Cylohexaned	Range	Δ(ΔG)e Average	Per –CH2–
Ile → Val (33)	25.8	−0.80	−0.88	−2.3 to 0.1	−1.1 ± 0.6	−1.1 ± 0.6
Val → Ala (39)	49.0	−1.24	−2.23	−4.9 to 0	−2.4 ± 1.1	−1.2 ± 0.5
Ile → Ala (26)	74.8	−2.04	−3.11	−5.4 to 0.7	−3.1 ± 1.2	−1.0 ± 0.4
Leu → Ala (40)	74.5	−1.90	−3.11	−6.2 to 0.2	−3.2 ± 1.2	−1.1 ± 0.4

^aFrom [13].

^bFrom [100].

^cIn kcal mol⁻¹ [101].

^dIn kcal mol⁻¹ [102].

^eIn kcal mol⁻¹. The negative values indicate a decrease in stability.