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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Mol Microbiol. 2014 Jul 6;93(3):415–425. doi: 10.1111/mmi.12665

Fig. 3. Delineation of the CupB5 signal that activates alginate production.

Fig. 3

(A) CupB5 variants with C-terminal truncations were cloned into pHERD20T, expressed in strain PAO1, and alginate production following growth in the presence of 1% L-arabinose was assayed. Proteins containing CupB5 residues 1–585 and 1–491 contain the complete GYYYTVV491 sequence motif. The remaining proteins contain only a portion of this motif. (B) Western-blot detection using anti-HA monoclonal antibody of truncated CupB5 variants.