Skip to main content
. 2014 Aug;184(8):2156–2162. doi: 10.1016/j.ajpath.2014.04.012

Figure 3.

Figure 3

Immunohistochemical staining and automated analysis output. Gleason score 3 + 4 = 7 negative control with only secondary antibody staining (A) and the resulting scoring output (E) from the Nuclear Image Analysis algorithm version 9.1 (Aperio). The lines drawn on each section are examples of areas chosen to be anaylzed by the Aperio software. Dashed lines represent areas to be removed from the analysis. A: Red arrows indicate dark nuclei that are still scored as negative because of lack of brown staining. C and D: Yellow arrows indicate specific nuclear localization of FASN. E–H: Blue output coloring from the algorithm indicates no nuclear staining, whereas yellow, orange, and red indicate mild, moderate, and strong nuclear staining, respectively. Gleason pattern 3 + 3 = 6 prostate cancer stained with anti-FASN antibody (B) and scoring output (F), Gleason pattern 3 + 4 = 7 prostate cancer stained with anti-FASN antibody (C) and scoring output (G), and Gleason pattern 4 + 4 = 8 prostate cancer stained with anti-FASN antibody (D) and scoring output (H).