Figure 8.

Nonhematopoietic β-arrestin-1 in sepsis-induced inflammation. Bone marrow chimeras were generated. The four groups of mice were subjected to cecal ligation and puncture (CLP) with a 16-guage needle and 12 hours later euthanized for sample collection. A–D: Cytokine levels as determined by ELISA in plasma (A), peritoneal fluid (B), spleen (C), and lung and liver lysates (D). E and F: Peritoneal neutrophil infiltration (E) and blood bacterial load (F) in septic mice are shown as total count and CFU/mL, respectively. Data in A–D are presented relative to wild type (WT) for each group. The chimeric nomenclature used is donor>recipient such that the chimeric mouse has the donor's hematopoietic cells and recipient's nonhematopoietic cells. Data are pooled from at least two independent experiments, except for the KO>KO group. n = 8 to 21, WT>WT, WT>KO, and KO>WT; n = 5, KO>KO. Error bars denote SEM. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 using Student's t-test. CFU, colony-forming units; KO, knockout.