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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2014 Jun 9;73:299–307. doi: 10.1016/j.freeradbiomed.2014.06.001

FIGURE 2. CuZnSOD nanozymes scavenge superoxide.

FIGURE 2

(A) Representative EPR spectra of the O2•− sensitive CMH spin probe in cell-free samples treated with vehicle, native CuZnSOD protein, reducible cl-nanozyme, or non-reducible cl-nanozyme. Superoxide was generated in these cell-free samples by hypoxanthine and xanthine oxidase. (a.u. – arbitrary units). (B) Summary EPR spectroscopy data showing CMH spectra amplitude, which is directly proportional to the amount of O2•− in the sample, in hypoxanthine/xanthine oxidase-containing samples treated with vehicle, native CuZnSOD protein, non-crosslinked nanozyme, reducible cl-nanozyme, or non-reducible cl-nanozyme (n = 4–5 per group). Data represent mean ± SEM. * P < 0.05 vs. vehicle.