FIGURE 5. Non-reducible cl-nanozyme delivers active CuZnSOD protein most efficiently to neurons in culture.
(A) Representative Western blot and summary quantification from lysates of CATH.a neurons treated (3 hours) with vehicle, native CuZnSOD protein, non-crosslinked nanozyme, reducible cl-nanozyme, or non-reducible cl-nanozyme (400U/mL of CuZnSOD was used for all CuZnSOD treatments). *P < 0.05 vs. vehicle. (B) Summary data showing SOD activity in CATH.a neurons incubated for 3 hours with the same treatments listed above (A). n = 4–5 separate neuronal cultures per group. Data represent mean ± SEM. * P < 0.05 vs. vehicle.