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Anticancer efficacy of polyarginine in a subcutaneous B16 murine melanoma model. Toll-like receptor 4 (TLR4)+/+ and TLR4−/− C57BL/6 mice received subcutaneous injection of phosphate buffered saline (PBS), polyarginine or lipopolysacharide (LPS). Tumor volume (a, n = 6) and survival (b, n = 10) were recorded. Data are expressed as mean ± SD. Statistical significance was measured by comparing the polyarginine or LPS TLR4+/+ mice with the PBS TLR4+/+ mice. *P < 0.05.
