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. Author manuscript; available in PMC: 2014 Aug 13.
Published in final edited form as: Nature. 2014 Jan 15;506(7487):240–244. doi: 10.1038/nature12883

Extended Data Figure 2. Multi-organ infiltration with blasts and dysplastic cells and myeloid differentiation block in βCat(ex3)osb mice.

Extended Data Figure 2

a, Blast infiltration (solid arrows) and neutrophil hypersegmentation (open arrow and magnified panels) in the blood of βCat(ex3)osb mice. Images at 40x or 100x. b, Blast infiltration (solid arrows) and micro-megakaryocytes with hyperchromatic nuclei (white arrows) in the bone marrow of βCat(ex3)osb mice. Images at 60X. c, Blast infiltration (solid arrows and magnified panel) and presence of dysplastic megakaryocytes (yellow arrows and magnified panel) in the spleen of βCat(ex3)osb mice. Image at 400X. d-f, Myeloperoxidase (MPO) staining of d, long bone e, spleen and f, liver showing massive invasion of myeloid cells. g, CD117 (C-kit) staining of bone sections showing CD117+ blasts in βCat(ex3)osb mice. h, CD13 staining of bone sections showing myeloid/monocytic infiltration in βCat(ex3)osb mice. In d-h images at 60X magnification. i-j, B-cell progenitors numbers in the i-j, bone marrow, k, spleen and l, lymph nodes.. m-t, Proportion of T-cells. u, Lack of myeloid cell differentiation in βCat(ex3)osb bone marrow cells following treatment with cytokines. v-x, Percentage of immature myeloid cells in ex vivo bone marrow cultures treated with cytokines. y. Robertsonian translocation between chromosomes 1 and 19 in 2 of 30 metaphases of the spleen of an 18-day old βcat(ex3)osb mouse. Inset shows the same abnormality in another cell. z, Whole-exome sequencing of myeloid malignancies (CD11b+/Gr1+) from 3 cat(ex3)osb mice and 3 germline normal (tail) samples.

In (i-x) N=6, mice per group. *p < 0.05 versus WT. Results are mean ± SD and show a representative of five (i-t) or three (u-x) independent experiments.