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. 2014 Jul 31;5:179. doi: 10.3389/fphar.2014.00179

FIGURE 3.

FIGURE 3

(A) GGsTop augments LLF glutathione in the presence of IL-13. Total Glutathione concentration (μM/ml) was measured in LLF at the end of the IL-13 experiment as described in “Materials and Methods” mice with the saline treated mice as control (0.4 μM/ml, n = 3). The glutathione concentration was similar to that of control after treatment with GGsTop (n = 3) and IL-13 (n = 3). It was significantly increased fourfold (1.6 μM/ml) in mice treated with GGsTop along with IL-13 (***p < 0.01, n = 3). (B–D) The IL-13 proinflammatory response is unaffected by GGsTop as IL-33 messenger RNA expression (B), assessed by qRT-PCR in total RNA isolated from lung as described in “Materials and Methods”, is induced sixfold by IL-13 treatment alone (n = 3) and with GGsTop (n = 3), and an eosinophilic infiltrate predominates with IL-13 alone (C) and with GGsTop (D) as described by Lowry et al. (2008).