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. Author manuscript; available in PMC: 2014 Jul 31.
Published in final edited form as: Nat Protoc. 2012 Apr 26;7(5):959–969. doi: 10.1038/nprot.2012.033

Figure 1.

Figure 1

Experimental set-up for analysis of metaphase spindle micromechanics. (a) Schematic of the system. A pair of microneedles is held by micromanipulators mounted on an inverted microscope. The CSF-arrested extract containing metaphase spindles is placed in a sample chamber. Confocal fluorescence imaging is used for visualizing chromosomes and spindle microtubules. Brigit field imaging is used for tracking motion of the microneedle tips. Photographs of a side view (b) and a top view (c) of the set-up around the sample chamber are presented.