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RT-PCR of select ldt genes, performed on RNA purified from exponential-phase cell cultures. Lane 1, DNA size markers (bp); lanes 2 and 3, MSMEG_0223, class 4 (ldtE), 295 bp; lanes 4 and 5, MSMEG_0674, class 6 (ldtG), 248 bp; lanes 6 and 7,: MSMEG_3528, class 1, (ldtA) 278 bp. Reactions in lanes 2, 4 and 6 included reverse transcriptase, whilst the control reactions in lanes 3, 5 and 7 lacked the enzyme.
