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. 2014 Aug 1;127(15):3240–3256. doi: 10.1242/jcs.141556

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Matn2 expression in proliferating and differentiating C2 myoblasts. (A–E) Phase-contrast images (A) and double immunofluorescence for Matn2 and other markers (B–E) of myoblast cultures differentiating to multinucleated myotubes (mmt) in differentiation medium. Desmin (B) and α-actinin staining (C) demonstrates the progress of differentiation. mb, myoblast; mt, myotube. (D) Partial colocalization of Matn2 with laminin-111 (Lam) and fibronectin (Fn). (E) Granular Matn2 deposition (asterisk) by proliferating myoblasts (day 0). Arrows mark the vinculin-positive focal adhesions left behind upon cell movement. Matn2 filaments are linked to the cells at paxillin-positive focal adhesions (arrowhead) on day 4. Separate images for Matn2 alone or for other markers and DAPI are shown above the main images in B, D and E. The area outlined in white in E is shown at higher magnification below the main image. (F) Comparison of mRNA levels for Matn2 and MRFs by northern hybridization. (G) Immunoblot analysis of Matn2 that was secreted daily into the differentiation medium. t, trimer; d, dimer; m, monomer.