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. 2014 Aug 1;127(15):3240–3256. doi: 10.1242/jcs.141556

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — The effect of TGF-β1 on the differentiation of Ctrl, sh3 and sh3-res2 myoblasts. Cells were cultured in differentiation medium with and without TGF-β1. (A) Immunofluorescent staining for α-actinin on day 3 of differentiation. (B) Smad1/5/8 phosphorylation during the differentiation of the control cell line was assessed by immunoblotting. (C) Western blot analysis of three pooled samples for Matn2, Smad4 and p21 expression. Cytoskeletal β-actin served as a loading control. t, trimer; d, dimer; m, monomer. (D) QRT-PCR of three pooled parallel cultures using the SYBR green protocol.