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. 2014 Aug 1;127(15):3240–3256. doi: 10.1242/jcs.141556

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 8. — The effect of Matn2 on marker gene expression during myoblast differentiation. (A) Impaired regeneration at day 28 in Matn2^−/− mice. Necrotic myofibers accumulate, which attracts numerous CD45-positive inflammatory cells (arrow), leading to increased fibronectin and collagen-3 deposition (magnified insets). (B) QRT-PCR analysis of marker gene expression in ex vivo differentiating myoblast cultures derived from Matn2^−/− and Matn2^+/+ newborn mice, performed using the SybrGreen protocol. Results show the mean±s.e.m. (C) Immunoblot analysis of integrin α5 in limbs of E16.5 embryos and in wild-type (WT) and Matn2^−/− (KO) primary myoblast cultures. (D) FAK detection by immunoblotting in myoblast cultures differentiating ex vivo. For C and D, the proteins were quantified by densitometry. (E) A model of myoblast differentiation. Regulatory interactions linking Matn2 expression to myoblast differentiation are illustrated schematically. Red arrows indicate activation processes affected directly or indirectly by Matn2 signaling. BM, basement membrane; Fn, fibronectin.