Fig. 4.
The ER transport of dendritic GABAB1 is controlled by multiple components. (A) Percentage recovery of GABAB1–EGFP for control samples or after treatment with 0.1 µg/µl cycloheximide (CHX) for 90 min. n = 4 and 5 neurons, respectively. (B) Same as above for the t1/2. (C,D) Same as in A,B for neurons under control conditions or treated with 100 µM nocodazole for 4 h (Noco High), 100 nM nocodazole for 5–70 min (Noco Low), 125 nM TSA for 30 min or 5 µM cytochalasin D for 2 h (Cyto D). n = 28, 9, 8, 20 and 4 neurons, respectively. At least three independent experiments were performed per condition. (E,F) Same as in A,B for neurons transfected with ER–RFP under control conditions or following treatment with 100 µM nocodazole for 4 h (ER–RFP+Noco). n = 4 and 7 neurons, respectively. (G,H) Same as in A,B for neurons transfected with Cytb5–GFP under control conditions or following treatment with 100 µM nocodazole for 4 h (Cytb5–GFP+Noco). n = 5 and 6 neurons, respectively. (I,J) Same as in A,B for neurons treated with 0.5 µg/ml BFA for 20 h or 0.5 µg/ml BFA for 20 h+100 µM nocodazole for 4 h (BFA+Noco). n = 3 and 4 neurons, respectively. All data show the mean±s.e.m.; *P<0.05; **P<0.01; n.s., non-significant; Student's t-test.