. Author manuscript; available in PMC: 2014 Jul 31.

Published in final edited form as: Nat Protoc. 2014 Jan 2;9(1):223–231. doi: 10.1038/nprot.2014.013

Table 1.

Analysis of spore viability and segregation of sister centromeres during meiosis I²¹.

Strain	mat1 alleles	Ectopic mat-Pc	P-factor addition	Mutation	Induction condition	Spore viability	Reductional anaphase I
JG12226 × JG15458	P/M	-	-	-	25°C	93%	99%
JG16022	M/M	-	-	pat1-as2	25°C 1-NM-PP1	56%	44%
JG16022	M/M	-	-	pat1-as2	34°C	32%	30%
JG16022	M/M	-	P-factor	pat1-as2	25°C 1-NM-PP1	81%	90%
JG16022	M/M	-	P-factor	pat1-as2	34°C	74%	85%
JG16113	M/M	mat-Pc	-	pat1-as2	25°C 1-NM-PP1	85%	96%
JG16113	M/M	mat-Pc	-	pat1-as2	34°C	78%	93%