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. Author manuscript; available in PMC: 2014 Jul 31.
Published in final edited form as: J Immunol. 2008 Apr 15;180(8):5636–5644. doi: 10.4049/jimmunol.180.8.5636

FIGURE 3.

FIGURE 3

Inhibition of the LPS-induced production of IL-8 decreased the TEM of human neutrophils. Supernatants from the Tyrphostin A9-treated (Tyr A9) and Pyk2 mutant-overexpressing (AAV-Pyk2MT) (A) or Pyk2-specific siRNA-treated (Pyk2 siRNA) and nontargeting siRNA (NT siRNA) (B) HUVEC cells were stimulated with LPS (100 ng/ml) and tested for their ability to induce neutrophil migration using a TEM assay.*, p < 0.05 vs the vehicle control. **, p < 0.05 compared with the AAV vector control (AAV-VC). ***, p < 0.05 vs the nontargeting siRNA control. C, HUVEC pretreated with various concentrations of polymyxin B were stimulated with 100 ng/ml LPS. The IL-8 content in the supernatants was measured after 24 h of stimulation. *, p < 0.05 vs the LPS control. D, Supernatants from untreated or LPS-treated HUVEC pretreated with IL-8 blocking Ab or an isotype control were also tested for their ability to induce neutrophil migration across the endothelium using the TEM assay. *, p < 0.05 vs the isotype control. Data represent mean ± SD of three independent experiments.