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. Author manuscript; available in PMC: 2014 Jul 31.
Published in final edited form as: Chemistry. 2011 Feb 8;17(10):2867–2873. doi: 10.1002/chem.201002848

Figure 2.

Figure 2

Three batches of particles containing allele-specific probes P1 and P3 (5’-CCTGGGAAAGT(C/A)CCCTCAACA-3’, alternatives shown in parentheses) form perfectly matched (PM) or mismatched (MM) duplexes on either end of a particle when they hybridize, respectively, with synthetic DNA targets T1 (a), T3 (b), and a 50/50 mixture of T1 and T3 (c), simulating three possible genotypes. A time-dependent difference in fluorescence retention ratio, Δ, across each particle is generated when subjected to an alkaline condition (pH 11.20 phosphate buffer). Δ curves for each hybridization scheme are shown in (d) and the peak values Δm are found for a mix of particles and plotted in a histogram (e). Δm fall into natural groups for each hybridization scheme. Scale bars are 100 μm.