Table 1. Global description of the primates samples and overall PCR, isolation and VP1 sequencing results.
Species | n | PCR Pos N (%) | Isolation (PCR-positive samples) N | Isolation (PCR-negative samples) Na | combined PCR and isolation N (%) | VP1 sequencing Nb | |
Wild | Chimpanzees | 96 | 10 (10.4) | 0 | ND | 10 (10.4) | 6 |
gorillas | 403 | 1 (0.25) | 0 | ND | 1 (0.25) | 1 | |
monkeys | 17 | 7 (41.2) | 0 | ND | 7 (41.2) | 4 | |
Total | 516 | 18 (3.5) | 0 | n/a | 18 (3.5) | 11 | |
Captive | Chimpanzees | 42 | 6c (14.3) | 1 | 3 | 9 (21.4) | 9 |
gorillas | 17 | 3d (17.6) | 0 | 1 | 4 (23.5) | 5 | |
monkeys | 40 | 6 (15.0) | 1 | 1 | 7 (17.5) | 2 | |
Total | 99 | 15 (15.2) | 2 | 5 | 20 (20.2) | 16 | |
Grand Total | 615 | 33 (5.4) | 2 | n/a | 38 (6.2) | 27 |
, ND, not done; n/a, not applicable.
, Overall 12 VP1 amplicons showed very low signal insufficient for sequencing reactions. However, 11 of the corresponding viruses could be sequenced in the 5′UTR region while the remaining one was refractory to 5′UTR amplification but showed a divergent 3Dpol sequence (GenBank n° KF648605 and KF648607).
, Includes one sample with a mixture of CV-A13 and an EV-A.
, Includes one sample with a mixture of EV-A71 and EV-B82.