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. 2014 Jul 31;8(7):e3052. doi: 10.1371/journal.pntd.0003052

Table 1. Global description of the primates samples and overall PCR, isolation and VP1 sequencing results.

Species n PCR Pos N (%) Isolation (PCR-positive samples) N Isolation (PCR-negative samples) Na combined PCR and isolation N (%) VP1 sequencing Nb
Wild Chimpanzees 96 10 (10.4) 0 ND 10 (10.4) 6
gorillas 403 1 (0.25) 0 ND 1 (0.25) 1
monkeys 17 7 (41.2) 0 ND 7 (41.2) 4
Total 516 18 (3.5) 0 n/a 18 (3.5) 11
Captive Chimpanzees 42 6c (14.3) 1 3 9 (21.4) 9
gorillas 17 3d (17.6) 0 1 4 (23.5) 5
monkeys 40 6 (15.0) 1 1 7 (17.5) 2
Total 99 15 (15.2) 2 5 20 (20.2) 16
Grand Total 615 33 (5.4) 2 n/a 38 (6.2) 27
a

, ND, not done; n/a, not applicable.

b

, Overall 12 VP1 amplicons showed very low signal insufficient for sequencing reactions. However, 11 of the corresponding viruses could be sequenced in the 5′UTR region while the remaining one was refractory to 5′UTR amplification but showed a divergent 3Dpol sequence (GenBank n° KF648605 and KF648607).

c

, Includes one sample with a mixture of CV-A13 and an EV-A.

d

, Includes one sample with a mixture of EV-A71 and EV-B82.