Table 2. Identification of enterovirus types and species in captive and wild non-human primates according to their species.
Virusesa | Primate Hosts | Isolation | Highest identity scorec (%) | Identificationd | GenBank accession no. | ||||
Speciesb | living setting | prototype | nt | aa | virus type | virus species | |||
Chimpanzees | |||||||||
CHB2* | Pan t. troglodytes | wild | EV-A76 | 84.5 | 93.0 | EV-A76 | EV-A | KF541633 | |
CHB5* | Pan t. troglodytes | wild | EV-A76 | 86.2 | 96.0 | EV-A76 | EV-A | KF541631 | |
CHB6 | Pan t. troglodytes | wild | EV-A76 | 85.6 | 97.0 | EV-A76 | EV-A | KF541649 | |
CHB7 | Pan t. troglodytes | wild | EV-A76 | 85.3 | 94.9 | EV-A76 | EV-A | KF541648 | |
CHB8* | Pan t. troglodytes | wild | EV-A76 | 85.9 | 95.0 | EV-A76 | EV-A | KF541632 | |
CHE20 | Pan t. troglodytes | wild | EV-J103 | 61.1 | 66.4 | EV-J121 | EV-J | KF541639 | |
Gorillas | |||||||||
GOJ01* | Gorilla gorilla | wild | EV-A92 | 68.8 | 69.0 | potential new type | EV-A | KF541630 | |
Monkeys | |||||||||
RCMH03 | red-capped mangabey | wild | SV6 | 57.8 | 52.2 | EV-123 | candidate new | KF541637 | |
RCMH05 | red-capped mangabey | wild | SV6 | 55.6 | 53.2 | EV-122 | candidate new | KF541636 | |
RCMH06 | Red-capped mangabey | wild | SV6 | 56.6 | 53.2 | EV-122 | candidate new | KF541638 | |
RCMH07 | Mandrillus sphinx | wild | SV6 | 57.9 | 52.2 | EV-123 | candidate new | KF541635 | |
Chimpanzees | |||||||||
Z004 | chimpanzee | captive | HEp-2c | CV-A13 | 73.0 | 87.7 | CV-A13 | EV-C | KF541646 |
Z033* | Pan t. troglodytes | captive | CV-A13 | 69.0 | 87.3 | CV-A13 | EV-C | KF541627 | |
Z034* | Pan t. troglodytes | captive | CV-A13 | 69.3 | 87.3 | CV-A13 | EV-C | KF541628 | |
Z035* | Pan t. troglodytes | captive | CV-A13 | 69.3 | 87.3 | CV-A13 | EV-C | KF541629 | |
Z036 | Pan t. troglodytes | captive | HEp-2c | CV-A13 | 73.3 | 87.7 | CV-A13 | EV-C | KF541645 |
Z108-1& | Pan t. troglodytes | captive | HEp-2c | CV-A13 | 73.3 | 87.7 | CV-A13 | EV-C | KF541647 |
Z046 | Pan t. troglodytes | captive | EV-A119 | 95.7 | 98.7 | EV-A119 | EV-A | KF541634 | |
Z055 | Pan t. troglodytes | captive | RD | E-29 | 80.6 | 95.9 | E-29 | EV-B | KF541644 |
Z110* | Pan t. troglodytes | captive | E-15 | 79.8 | 95.4 | E-15 | EV-B | KF541626 | |
Gorillas | |||||||||
Z052 | Gorilla gorilla | captive | CV-A24 | 78.3 | 89.5 | CV-A24 | EV-C | KF541640 | |
Z097 | Gorilla gorilla | captive | HEp-2c | E-15 | 78.9 | 90.8 | E-15 | EV-B | KF541641 |
Z098-1*& | Gorilla gorilla | captive | EV-B82 | 81.6 | 91.5 | EV-B82 | EV-B | KF541625 | |
Z098-2& | Gorilla gorilla | captive | EV-A71 | 79.4 | 94.3 | EV-A71 | EV-A | KF700265 | |
Z099 | Gorilla gorilla | captive | EV-A71 | 79.4 | 94.3 | EV-A71 | EV-A | KF700264 | |
Monkeys | |||||||||
Z057 | Mandrillus sphinx | captive | HEp-2c and Vero | SA5 | 82.1 | 92.9 | SA5 | EV-B | KF541643 |
Z088 | Mandrillus sphinx | captive | HEp-2c and RD | E-29 | 80.5 | 95.6 | E-29 | EV-B | KF541642 |
Viruses from wild primates are abbreviated in the following format: two letters code standing for the primate species identified in the field (CH, chimpanzee; GO, gorilla; RCM, red-capped mangabey), followed by one later (from B to J) corresponding to the round of sample collection on the field and the serial number of the stool sample. Viruses from captive primates are abbreviated using the letter “Z” standing for Zoo, followed by the serial number of the stool sample. For samples containing 2 viruses, “−1” and “−2” were used to distinguish among each others. Viruses specified with an asterisk (*) have been typed using partial VP1 sequences.
Species designated with scientific names were confirmed by the analysis of a portion of simian mitochondrial DNA sequences; Pan t. troglodytes, Pan troglodytes troglodytes.
nt, nucleotides; aa, amino acids ; SA5, simian picornavirus 17.
Candidates new types discovered in this study are highlighted in bold.
Chimpanzee sample Z108 contained two isolates Z108-1 (CV-A13) and a potentially divergent EV-A (Z108-2) which could be sequenced only in the 3Dpol region; the gorilla sample Z098 contained two isolates Z098-1 (EV-B82) and Z098-2 (EV-A71).