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. 2014 Jul 31;8(7):e3051. doi: 10.1371/journal.pntd.0003051

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© 2014 Ishida et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Purified IgG from SmHsf1-immunized rabbit bleeds (lanes 1–4) or pre-immune serum (lane 5) were used in a Western blot to test for reactivity against bacterially expressed recombinant proteins and cercarial extract (lane 1), 1 µg MBP negative control (lane 2), 1 µg MBP- SmHsf1 fusion protein (lane 3), 7 µg MBP- SmHsf1 fusion protein (lanes 4 & 5), 7 µg cercarial extract.