Figure 2. Effect of auranofin on parasite viability and plaque formation.

A. Auranofin significantly decreased the rate of infection after 24(three independent experiments were conducted with triplicates, and at least 100 cells were counted. The number of infected cells was determined per each high power field by light microscopy. The percentage of infection was calculated as the total number of infected cells divided by the total number of cells multiplied by 100). B. Viability of extracellular tachyzoites in complete medium at 18 hrs (overnight) using ATP assay. RLU: relative luminescence units. C. The number of plaques observed at day 7 post infection was significantly different. Observations from 3 independent experiments are presented (n = total number of observations). Data presented as mean ±SD.