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. 2014 Jul 31;9(7):e102237. doi: 10.1371/journal.pone.0102237

Figure 1. Genomic Southern blot.

Figure 1

Autoradiograph of genomic southern blot using a P32 labelled probe of ADH-1 target region from F. vesca accession PAWT. In lanes 1–4, genomic DNA from FRA1223 (F. nilgerrensis), FRA377 (F. iinumae), U2A (F. vesca) and YW (F. vesca), respectively, were digested with MscI, which has one expected cut site in the target DNA. In lanes 5–8 the above accessions were digested with HindIII and those in lanes 9–12 were digested with XbaI, both with no expected cut sites in the target DNA.