Table 2. Summary of cap structures that were cross-validated by 2D-TLC and mass spectroscopy.
5′end structure | Name | Known RNAs with thismodification in human | Biological function ofthis RNA | sRNA fraction with thismodification (nt) |
2,2,7mGp | N2,N2,7-trimethylguanosine monophosphate | snoRNA | rRNA modification | 20 nt–200 nt |
2,2mGp | N2,N2 dimethylguanosine monophosphate | tRNA, rRNA | 20 nt–200 nt | |
2,7mGp | N2,7-dimethylguanosine monphosphate | ND | 20 nt–50 nt | |
7mGp | 7-methylguanosine monophosphate | tRNA, rRNA, mRNA | 20 nt–50 nt 100 nt | |
2mGp | 2-methylguanosine monophosphate | tRNA | 20 nt–200 nt | |
1mGp | 1-methylguanosine monophosphate | tRNA, snRNA | translation | 20 nt–50 nt 100 nt |
Cmp | 2′-O-methylcytidine monophosphate | tRNA, rRNA, snRNA, mRNA | translation, transcription | 20 nt–200 nt |
Gp | 5′-guanosine monophosphate | All | 20 nt–200 nt | |
Cp | 5′-cytosine monophosphate | All | 20 nt–200 nt | |
Ap | 5′-adenosine monophosphate | All | 20 nt–200 nt | |
Up | 5′-uridine monophosphate | All | 20 nt–200 nt |
*: Unique to this fraction.
**: First time to be reported in mammals.