Figure 4. Western blots with polyclonal pAbs.

The recombinant (rPP13) wild type PP13, its truncated variant (DelT₂₂₁) and the native molecule (nPP13) purified from the placenta were separated by 15% SDS PAGE under non-reducing (A) or reducing (B) conditions, electro transferred to nitrocellulose and reacted with rabbit pAbs to PP13 (J67), pre-immune rabbit IgG (Jo) or without antibody (HRP negative control) followed by incubation with goat anti rabbit IgG conjugated with HRP. A single band is shown for the native and truncated PP13 in both reduced and non-reduced conditions. The recombinant wild type generates a single band in reduced conditions but in non-reduced conditions dimer, trimer and tetramer PP13 are revealed. The recombinant PP13 is heavier than the native protein due to the his-tag and extra tail sequence in the recombinant protein.