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. 2014 Jul 31;9(7):e101276. doi: 10.1371/journal.pone.0101276

Figure 2. FTY720 increases smad-1 mediated osteogenic differentiation of progenitor cells.

Figure 2

(A) Western blot analysis of BMSCs reveals increase in smad-1 phosphorylation when treated with FTY720 compared to BMP-2. (B–C) Flow cytometry for phosphor-smad in murine MSCs reveals FTY720 treatment enhances BMP-2 induced smad-1 activity. (D) FTY720 enhances mineralization of progenitor cells, shown by Alizarin Red and (E) qPCR shows increase in Runx2 mRNA in progenitor cells when treated with FTY720, shown as fold change over SFM. (** p<0.01 & *p<0.05).