Figure 1. Wild-type and mutant endoglin proteins show intracellular colocalisation.

CHO cells were co-transfected with equal amounts of endoglin^wt (ECFP-tagged) and endoglin missense mutant (EYFP-tagged) expression constructs as indicated. The localisation of expressed proteins was analysed by fluorescence microscopy. Irrespective of the fluorophore colours, mutant proteins are shown in red and endoglin^wt is shown in green. Mutants up to amino acid position 413 are retained inside the cell, while endoglin^wt shows membrane localisation, but also enhanced intracellular localisation when co-expressed with intracellularly retained mutants, as seen in yellow in the merged image.