Figure 2. Endoglin missense mutants colocalise with the rER resident protein Calnexin.

HMEC-1 cells were transfected with EYFP-tagged endoglin missense mutant constructs, permeabilized, and antibody stained against the rER resident chaperone protein calnexin. Two mutant proteins, G52V and G413V, were exemplarily investigated, with G52V resembling the lowest amino acid position and G413V representing the highest amino acid of the missense mutants here analysed that are intracellularly retained.