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. 2014 Jul 31;9(7):e103096. doi: 10.1371/journal.pone.0103096

Figure 1. Schematics of box C/D ribonucleoprotein particles (RNPs) and chimera RNA used in co-expression studies.

Figure 1

A) Eukaryotic box C/D RNPs comprise Nop56, Nop58, fibrillarin (FIB), 15.5 K (Snu13p in yeast), and a box C/D RNA. In archaea, Nop56 and Nop58 have a single homolog, Nop5, and 15.5 K has a homolog, L7Ae. The protein assembly model is based on architecture revealed by archaeal box C/D RNP crystal structures. B) Construction of the tRNA-box C/D RNA chimera. tRNA is divided into two parts (part I and part II) that flank the sequence encoding the box C/D RNA.