Figure 6. The relative gene expressions of hp0138, hp0140, and hp1222 in H. pylori 26695 wild type grown in a variety of carbon sources (5 mM glucose, 10 mM L-lactate, 10 mM D-lactate, 2.5 mM glucose+5 mM L-lactate, 2.5 mM glucose+5 mM D-lactate, or 5 mM L-lactate+5 mM D-lactate).

Real-time quantitative PCRs were performed in 7300 Real-Time PCR System (Applied Biosystems) using Taqman-designed primers and probes (Life technologies), and the data were analyzed by comparative Ct (ΔΔCt) method with 16S rRNA as the reference gene. All the quantifications were performed in triplicate and were shown as the means ± SD.