Table 1. Bacterial strains and plasmids used in this study.
Strains or plasmids | Characteristics or genotypes* | Reference or source |
Helicobacter pylori | ||
26695 | H. pylori wild-type strain, parental strain for mutants in this study | ATCC[9] |
Δ140 | hp0140–0141 disrupted derivative of 26695, Cmr | This study |
Δ138 | hp0137–0139 disrupted derivative of 26695, Cmr | This study |
Δ1222 | hp1222 disrupted derivative of 26695, Kanr | This study |
Δ138/Δ1222 | hp0137–0139 and hp1222 disrupted derivative of 26695, Cmr, Kanr | This study |
J99 | H. pylori wild-type strain | [7] |
NCTC 11637 | H. pylori wild-type strain, identical to CCUG 17874 | [39] |
G27 | H. pylori wild-type strain | [40] |
TN2GF4 | H. pylori wild-type strain | [41] |
SS1 (Sydney strain) | H. pylori wild-type strain (mouse-adapted) | [42] |
Campylobacter jejuni ATCC 33292 | C. jejuni wild-type strain | ATCC |
Escherichia coli DH5α | Host strain used for plasmid construction | Takara |
Plasmids | ||
pCR Blunt II TOPO | Blunt-end cloning vector; Zeor, Kanr | Invitrogen |
pGH32 | pT7Blue vector with chloramphenicol resistance gene (cat); Ampr, Cmr | lab collection |
pGH84 | pT7Blue vector with kanamycin resistance gene (aph3); Ampr, Kanr | lab collection |
pSI01 | TOPO vector with 4.8 kb fragment containing hp0140-hp0141 | This study |
pSI02 | pSI01 derivative with hp0140-hp0141::cat | This study |
pSI03 | TOPO vector with 2.8 kb fragment containing hp1222. | This study |
pSI04 | pSI03 derivative with hp1222::aph3 | This study |
pSI05 | TOPO vector with 2.8 kb fragment containing hp0137-hp0139 | This study |
pSI06 | pSI05 derivative with hp0137–0139::cat | This study |
*Cmr, chloramphenicol resistance; Kanr, kanamycin resistance; Zeor, zeocin resistance; Ampr, ampicillin resistance.