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. 2014 Jul 31;9(7):e103506. doi: 10.1371/journal.pone.0103506

Table 1. Bacterial strains and plasmids used in this study.

Strains or plasmids Characteristics or genotypes* Reference or source
Helicobacter pylori
26695 H. pylori wild-type strain, parental strain for mutants in this study ATCC[9]
 Δ140 hp0140–0141 disrupted derivative of 26695, Cmr This study
 Δ138 hp0137–0139 disrupted derivative of 26695, Cmr This study
 Δ1222 hp1222 disrupted derivative of 26695, Kanr This study
 Δ138/Δ1222 hp0137–0139 and hp1222 disrupted derivative of 26695, Cmr, Kanr This study
J99 H. pylori wild-type strain [7]
NCTC 11637 H. pylori wild-type strain, identical to CCUG 17874 [39]
G27 H. pylori wild-type strain [40]
TN2GF4 H. pylori wild-type strain [41]
SS1 (Sydney strain) H. pylori wild-type strain (mouse-adapted) [42]
Campylobacter jejuni ATCC 33292 C. jejuni wild-type strain ATCC
Escherichia coli DH5α Host strain used for plasmid construction Takara
Plasmids
pCR Blunt II TOPO Blunt-end cloning vector; Zeor, Kanr Invitrogen
pGH32 pT7Blue vector with chloramphenicol resistance gene (cat); Ampr, Cmr lab collection
pGH84 pT7Blue vector with kanamycin resistance gene (aph3); Ampr, Kanr lab collection
pSI01 TOPO vector with 4.8 kb fragment containing hp0140-hp0141 This study
pSI02 pSI01 derivative with hp0140-hp0141::cat This study
pSI03 TOPO vector with 2.8 kb fragment containing hp1222. This study
pSI04 pSI03 derivative with hp1222::aph3 This study
pSI05 TOPO vector with 2.8 kb fragment containing hp0137-hp0139 This study
pSI06 pSI05 derivative with hp0137–0139::cat This study

*Cmr, chloramphenicol resistance; Kanr, kanamycin resistance; Zeor, zeocin resistance; Ampr, ampicillin resistance.