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. 2014 Jul 31;9(7):e103012. doi: 10.1371/journal.pone.0103012

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© 2014 Mezzina et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Far-UV CD Spectra of PhaP_Az (7 µM) in 20 mM sodium phosphate buffer pH 7.3 containing 50mM NaCl and 1 mM DTT, at different GdmCl concentrations in the range of 0 to 5.8 M. (B) PhaP_Az chemical unfolding monitored by fluorescence spectroscopy and CD: (•) ANS fluorescence as a function of GdmCl (○) Molar ellipticity at 222 nm as a function of GdmCl concentration. (C) Fluorescence spectra of ANS binding to PhaP_Az (7 µM) treated with different concentrations of GdmCl in the range of 0 to 5.8 M.