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Transduction of SCCVII cells with an interleukin (IL)-12 lentiviral vector led to the isolation of clones that secrete IL-12 at various levels. (A) Secretion of IL-12 by clones when plated at a concentration of 106 cells/ml/4 hrs. (B) doubling time of IL-12-expressing and non-expressing SCCVII clones as calculated by loss of CFSE staining.
