Figure 4.

Effects of I/R and rhRLX on activity and expression of manganese-superoxide dismutases (MnSOD) and copper-zinc-superoxide dismutases (CuZnSOD) in kidney samples. Activity (A and B) and protein expression (C and D) of the SOD isoforms mitochondrial MnSOD and cytosolic CuZnSOD were measured in kidney homogenates of sham-operated rats (Sham) and rats that underwent 1-hr ischaemia and 6-hr reperfusion (IR) in the absence or presence of rhRLX (5 μg/kg, i.v.; Sham+RLX and IR+RLX). Each immunoblot is from a single experiment and is representative of three separate experiments. Densitometric analysis of the bands is expressed as relative optical density (O.D.), corrected for the corresponding β-actin contents and normalized using the related sham-operated band. Data are means ± SEM of three separate experiments for Western Blot and five animals/group for SOD activity. ⋆P < 0.05 versusIR.