Figure 5.

Effects of I/R and rhRLX on leucocyte accumulation in kidney samples. Myeloperoxidase (MPO) activity (A) and ICAM-1 expression (B) were measured in kidney homogenates of sham-operated rats (Sham) and rats that underwent 1-hr ischaemia and 6-hr reperfusion (IR) in the absence or presence of rhRLX (5 μg/kg, i.v.; Sham+RLX and IR+RLX). Each immunoblot is from a single experiment and is representative of three separate experiments. Densitometric analysis of the bands is expressed as relative optical density (O.D.), corrected for the corresponding β-actin contents and normalized using the related sham-operated band. Data are means ± SEM of three separate experiments for Western Blot and five animals/group for MPO. ⋆P < 0.05 versusIR.