Figure 5. ATF4 does not affect the proliferation or colony formation of ESCC cells.
(A) MTT assay of the proliferation of TE-1LM non-treated control (NC), TE-1LM-vector (Vector), and TE-1LM-ATF4 (ATF4) cells. The data represent the means ± S.D. of three independent experiments. (B) The effects of ATF4 on the proliferation of the cells as described above were also examined using a colony formation assay. The media was changed every 3 d. Cells were plated in triplicate, and the experiment was repeated three times. Representative wells are shown.